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Our Mission

"The VIB Protein Core offers solutions for producing mg to gram amounts of research grade proteins"

 

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Products

The Protein Core can provide you with some commonly used recombinant proteins. These proteins can be ordered directly from our online platform Connect. Most of these proteins are cytokines, antibodies, …
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Team

Our team doesn't only work with proteins but thinks like proteins
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VIB Protein Core

About the Core

The VIB Protein Core services include scale-up of microbial cultures up to 6 or 20 L., mammalian cell culture up to 12 L., and downstream processing and purification adapted to those scales. The deliverable is the end product of your process (usually a protein) and documentation that each step is carried out to the state of the art. In order to rapidly optimize expression analysis, the VIB Protein Core uses a technology platform for parallel expression analysis with numerous possible combinations, i.e. FastScreen. Expression optimization is offered in Escherichia coliPichia pastoris and mammalian cells (HEK293F, CHO). For expression in mammalian cells, the Core can use transient expression (in HEK293F cells) or can provide you with a stable cell line (HEK293F, CHO) expressing your protein of interest. Matrix-approach-based optimizations are also available for refolding of inclusion bodies, i.e. FastFold, and for purification process optimization, i.e. FastClean. Based on the FastScreen result, a Production can be performed. The FastClean can lead to a Purification strategy to make mg amounts of pure protein. Depending on the purpose of the pure protein; fluorescent labels, polymers, … can be attached by ConjugationProtein Analytics can be performed to guarantee the quality of the delivered protein. Some Protein Analytics are done in-house, others in collaboration with specialized groups or institutes. Not only the purity, concentration or LPS content is important but also the activity or affinity of a protein. Therefore the Core performs also different (cellular) Activity Assays and invests in technologies for Protein-Protein Interactions (Bio-Layer Interferometry, Octet Red). The VIB Protein Core invested also in the SEC/MALS/UV/RI technology to understand even better the folding and monomeric/oligomeric state of your protein in solution. The quality of the recombinant protein is important.

Beyond servicing, Research on new technologies and opportunities available on the market is done regularly by the Core. By the enormous interest in single-domain antibodies (also called nanobodies®), the VIB Protein Core has specialized in Nanobody® Production and Purification.

Contact Info

VIB Protein Core
Jurgen Haustraete
Technologiepark-Zwijnaarde 71
9052 Ghent
Belgium